Objectives: The aim of the research was to perform sequencing of 2 housekeeping genes of N. meningitidis (abcZ and fumC), isolated from patients, and to set phylogenetic relationships between investigated strains. Methods: We used 11 isolates of N. meningitidis (serogroup A- 1 isolate, serogroup B- 8 isolates, and serogroup C- 2 isolates). Detection of housekeeping genes as markers of phylogenetic relationship was carried out with the help of PCR. Sequencing reaction was performed based on Senger method. Amplification and sequencing protocols, allele information for multilocus sequence typing (MLST) were taken over the web- site http://pubmlst.org/neisseria/. Nucleotide sequences of abcZ and fumC genes of isolates analyzed were compared with the data in GenBank using BLAST (http://www.ncbi.nlm.nih.gov/BLAST) and Neisseria MLST database (http://pubmlst.org/neisseria). Results: Sequence analysis of abcZ gene of 11 N. meningitidis strains, isolated from patients, showed their west- european origin, and sequences of some strains (1, 308 and 325), admittedly, have indigenous origin- they differ greatly from alleles in the Internet database and while constructing evolutional dendrogramm perform separate cluster. Moreover, after these sequences were compared with those in database, similarity in the major nucleotide positions was found, but in general search no similar alleles were found. Strains 1, 308 and 325, isolated from patients, appear to be more old, and the rest isolates associated with each other according to the extend of sequences homology (more than 70% of homology) of analyzed fragments. While analyzing fumC gene of 7 N. meningitidis isolates, sequence analysis showed similarity to west- european alleles and to east allele (strain 275). Two fumC sequences (83 and 308 strains) fundamentally differ from other analyzed sequences, perform separate cluster and underlie the constructed phylogenetic tree, what suggests their earlier appearance (probably indigenous origin). So, both general and rare alleles fundamentally import the intraspecific allelic formation. Conclusions: Meningococcal population in the republic has heterogeneous structure. According to the analysis of abcZ and fumC genes sequences received one can say, that the population of meningococci in the country has both indigenous and west- european and east origin, which may be determinated by migration and genetic exchange between strains. High similarity (70% and more) of the received sequences to alleles in the Internet database suggests the occurrence of genetic replacement and mutations in abcZ and fumC genes, that are not studied yet in details and are not represented in the database. And similarity in 90% and more, probably, supposes that well- known alleles of Neisseria meningitidis acquire mutations.
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